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cfx 96 tm real time pcr instrument  (Bio-Rad)


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    Bio-Rad cfx 96 tm real time pcr instrument
    Cfx 96 Tm Real Time Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 28124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfx+96+touch+real+time+pcr+instrument/CFX96+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pm40597643-105-8-7
    Average 96 stars, based on 28124 article reviews
    cfx 96 tm real time pcr instrument - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Transcriptome analysis reveals the regulation of miR-19b on inflammation in bovine mammary epithelial cells.
    Article Snippet: MicroRNAs (miRNAs) are involved in various biological processes where they regulate the expression of mRNAs.. Bovine mammary epithelial cells (bMECs) are functional cells that mediate mammary inflammatory immunity.. Although numerous miRNAs regulate the function of bMECs, the role of miR-19b in bMECs has not been reported.

    Article Title: Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells
    Article Snippet: Briefly, the 1 μg total RNA in bMECs was reverse transcribed into cDNA using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara Biomedical Technology Co., Ltd., Beijing, China) following the manufacturer’s instructions. .. The 2×M5 HiPer SYBR Premix EsTaq (withTli RNaseH) fluorescence quantitative detection kit (Mei5 Biotechnology Co., Ltd., Beijing, China) detected the expression of DIE-mRNAs in transfected MAC-T cells using a CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA). ..

    Article Title: Bta-miR-199a-3p Inhibits LPS-Induced Inflammation in Bovine Mammary Epithelial Cells via the PI3K/AKT/NF-κB Signaling Pathway
    Article Snippet: The cDNA synthesis of bta-miR-199a-3p and related mRNA was performed using the PrimeScript TM RT Reagent Kit with gDNA Eraser (Takara, Beijing, China). .. Using this cDNA as a template, the expression levels of bta-miR-199a-3p and related mRNA in the cells were detected using a CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA) and 2 × M5 HiPer SYBR Premix EsTaq (with Tli RNaseH) (Mei5bio, Beijing, China) [ ]. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptome analysis reveals the regulation of miR-19b on inflammation in bovine mammary epithelial cells.
    Article Snippet: MicroRNAs (miRNAs) are involved in various biological processes where they regulate the expression of mRNAs.. Bovine mammary epithelial cells (bMECs) are functional cells that mediate mammary inflammatory immunity.. Although numerous miRNAs regulate the function of bMECs, the role of miR-19b in bMECs has not been reported.

    Article Title: Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells
    Article Snippet: Briefly, the 1 μg total RNA in bMECs was reverse transcribed into cDNA using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara Biomedical Technology Co., Ltd., Beijing, China) following the manufacturer’s instructions. .. The 2×M5 HiPer SYBR Premix EsTaq (withTli RNaseH) fluorescence quantitative detection kit (Mei5 Biotechnology Co., Ltd., Beijing, China) detected the expression of DIE-mRNAs in transfected MAC-T cells using a CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA). ..

    Article Title: Bta-miR-199a-3p Inhibits LPS-Induced Inflammation in Bovine Mammary Epithelial Cells via the PI3K/AKT/NF-κB Signaling Pathway
    Article Snippet: The cDNA synthesis of bta-miR-199a-3p and related mRNA was performed using the PrimeScript TM RT Reagent Kit with gDNA Eraser (Takara, Beijing, China). .. Using this cDNA as a template, the expression levels of bta-miR-199a-3p and related mRNA in the cells were detected using a CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA) and 2 × M5 HiPer SYBR Premix EsTaq (with Tli RNaseH) (Mei5bio, Beijing, China) [ ]. ..

    Article Title: Modular micro-PCR system for the onsite rapid diagnosis of COVID-19
    Article Snippet: .. Detection was performed using a BioRad CFX 96 TOUCH Real-time PCR instrument. ..

    Article Title: Alfalfa xenomiR-162 targets G protein subunit gamma 11 to regulate milk protein synthesis in bovine mammary epithelial cells
    Article Snippet: Complementary DNA (cDNA) was then synthesized using the PrimeScript RT reagent kit with the gDNA Eraser kit (Takara Biomedical Technology Co., Ltd, China) following the manufacturer’s protocol. .. Gene expression was assessed by quantitative polymerase chain reaction (qRT-PCR) using CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA) real-time fluorescence quantification system and the ChamQ Universal SYBR qRT-PCR Master Mix real-time fluorescence quantitative PCR kit (Vazyme Biotech Co., Ltd, Nanjing, China). .. Primer Premier 5.0 (Premier Biosoft, San Francisco, CA, USA) software was used to design qRT-PCR primers for its related genes ( ).

    Article Title: Modular micro-PCR system for the onsite rapid diagnosis of COVID-19
    Article Snippet: .. RESOLUTE 2.0 test was performed using 5μl of UTM per standard protocol in Table 4 and detected on a BioRad CFX 96 TOUCH Real-Time PCR Instrument. .. The analytical LoD for the RESOLUTE 2.0 was determined to be 10 RNA copies/μL for the RESOLUTE 2.0 E Gene and N Gene assays for the BioRad CFX96TM TOUCH Real-time PCR system.

    Article Title: Clinical, pathological, and genotypic analysis of infectious bronchitis virus in broiler chickens in the Abu Dhabi Emirate, United Arab Emirates
    Article Snippet: .. RT-qPCR analysis was conducted using the BioRad CFX 96 Touch Real-Time PCR Instrument (BioRad). .. The 464 bp hyper-variable region of the S1 gene was PCR amplified using the SuperScript TM III PlatinumTM One-Step RT-PCR Kit (Thermo Fisher, Waltham, MA, USA), following a previously described method ( ) with minor modifications.

    Fluorescence:

    Article Title: Transcriptome analysis reveals the regulation of miR-19b on inflammation in bovine mammary epithelial cells.
    Article Snippet: MicroRNAs (miRNAs) are involved in various biological processes where they regulate the expression of mRNAs.. Bovine mammary epithelial cells (bMECs) are functional cells that mediate mammary inflammatory immunity.. Although numerous miRNAs regulate the function of bMECs, the role of miR-19b in bMECs has not been reported.

    Article Title: Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells
    Article Snippet: Briefly, the 1 μg total RNA in bMECs was reverse transcribed into cDNA using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara Biomedical Technology Co., Ltd., Beijing, China) following the manufacturer’s instructions. .. The 2×M5 HiPer SYBR Premix EsTaq (withTli RNaseH) fluorescence quantitative detection kit (Mei5 Biotechnology Co., Ltd., Beijing, China) detected the expression of DIE-mRNAs in transfected MAC-T cells using a CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA). ..

    Article Title: Alfalfa xenomiR-162 targets G protein subunit gamma 11 to regulate milk protein synthesis in bovine mammary epithelial cells
    Article Snippet: Complementary DNA (cDNA) was then synthesized using the PrimeScript RT reagent kit with the gDNA Eraser kit (Takara Biomedical Technology Co., Ltd, China) following the manufacturer’s protocol. .. Gene expression was assessed by quantitative polymerase chain reaction (qRT-PCR) using CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA) real-time fluorescence quantification system and the ChamQ Universal SYBR qRT-PCR Master Mix real-time fluorescence quantitative PCR kit (Vazyme Biotech Co., Ltd, Nanjing, China). .. Primer Premier 5.0 (Premier Biosoft, San Francisco, CA, USA) software was used to design qRT-PCR primers for its related genes ( ).

    Transfection:

    Article Title: Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells
    Article Snippet: Briefly, the 1 μg total RNA in bMECs was reverse transcribed into cDNA using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara Biomedical Technology Co., Ltd., Beijing, China) following the manufacturer’s instructions. .. The 2×M5 HiPer SYBR Premix EsTaq (withTli RNaseH) fluorescence quantitative detection kit (Mei5 Biotechnology Co., Ltd., Beijing, China) detected the expression of DIE-mRNAs in transfected MAC-T cells using a CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA). ..

    Quantitative RT-PCR:

    Article Title: LncRNA HULIB promotes LPS induced inflammatory response in bovine mammary epithelial cells via PP2AB.
    Article Snippet: Bovine mastitis is regulated by genetic and environmental factors.. Long non-coding RNAs (LncRNAs), which regulate various biological processes (immune system and biological development), have been found to play a role in bovine mammary inflammation responses.. Here, a novel functional lncRNA, named lncRNA HULIB, was identified as a regulator during bovine mastitis. qRT-PCR and subcellular fractionation assays showed that lncRNA HULIB was significantly up-regulated in LPS-induced bMECs and was mainly localized in the cytoplasm.

    Article Title: Alfalfa xenomiR-162 targets G protein subunit gamma 11 to regulate milk protein synthesis in bovine mammary epithelial cells
    Article Snippet: Complementary DNA (cDNA) was then synthesized using the PrimeScript RT reagent kit with the gDNA Eraser kit (Takara Biomedical Technology Co., Ltd, China) following the manufacturer’s protocol. .. Gene expression was assessed by quantitative polymerase chain reaction (qRT-PCR) using CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA) real-time fluorescence quantification system and the ChamQ Universal SYBR qRT-PCR Master Mix real-time fluorescence quantitative PCR kit (Vazyme Biotech Co., Ltd, Nanjing, China). .. Primer Premier 5.0 (Premier Biosoft, San Francisco, CA, USA) software was used to design qRT-PCR primers for its related genes ( ).

    Article Title: Clinical, pathological, and genotypic analysis of infectious bronchitis virus in broiler chickens in the Abu Dhabi Emirate, United Arab Emirates
    Article Snippet: .. RT-qPCR analysis was conducted using the BioRad CFX 96 Touch Real-Time PCR Instrument (BioRad). .. The 464 bp hyper-variable region of the S1 gene was PCR amplified using the SuperScript TM III PlatinumTM One-Step RT-PCR Kit (Thermo Fisher, Waltham, MA, USA), following a previously described method ( ) with minor modifications.

    Gene Expression:

    Article Title: Alfalfa xenomiR-162 targets G protein subunit gamma 11 to regulate milk protein synthesis in bovine mammary epithelial cells
    Article Snippet: Complementary DNA (cDNA) was then synthesized using the PrimeScript RT reagent kit with the gDNA Eraser kit (Takara Biomedical Technology Co., Ltd, China) following the manufacturer’s protocol. .. Gene expression was assessed by quantitative polymerase chain reaction (qRT-PCR) using CFX-96 Touch Real-Time PCR instrument (BioRad, Hercules, CA, USA) real-time fluorescence quantification system and the ChamQ Universal SYBR qRT-PCR Master Mix real-time fluorescence quantitative PCR kit (Vazyme Biotech Co., Ltd, Nanjing, China). .. Primer Premier 5.0 (Premier Biosoft, San Francisco, CA, USA) software was used to design qRT-PCR primers for its related genes ( ).



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